The concern for safety, cost-cutting, eco-friendliness and
local availability is driving the quest to explore the abundant local plant
resources in developing countries. Researchers in these countries are now looking
inwards to make use of resources in their domain to solve their problems.
Recent works done in this regard are chronicled in this write up.
1. Punica
Granatum , (pomegranate): This was used for the staining of rat brain (Gharravi
et al., 2006).
2. Curcuma
longa: The crude ethanolic extract and column chromatographic fraction of this
local dye was used for the demonstration of collagen, muscle cells and red
blood cells (Awvioro, 2007). It was also used for staining of seminiferous
epithelium (Bassey et al., 2011).
3. Kola nut
(cola acuminata): This was used as eosin substitute to stain tissues (Shehu et
al., 2012).
4. Black
plum fruit (Syzygium cumini): This was used as histological stain for rat hepatic
tissue (Suabjakyong et al.,2011)
5. Extract
of butterfly pea flowers (Clitoria ternatea). This was used as histological
stain for blood cells (Suebkhumpet and Sotthibandhu, 2012).
6. Extract
of dry leaves of henna (Lawsonia intermis).
This was used as histological stain for angiospermic stem (Jan et al.
(2011).
7. Red beet
(Beta vulgaris) extract. This was used
as a histological stain for intestinal parasites (Al-Amura and co-researchers
(2012).
8. Black Mulberries. This was used as histological stain for
tissues (Tousson
and AL-Behbehani (2011).
9. Sorghum
bicolor. This was used as suitable
counterstain for haematoxylin in histological sections (Omoowo, 2014). Also
used for rat hippocampus cells demonstration
(Muhammed et al., 2016), liver and kidney (Benard et al., 2015) and brain
cells (Benard et al., 2015).
10. Baphia
nitida (Camwood). This was used as histological stain for dentine tissues
(Fasan,
1977).
11. Hibiscus
sabdariffa. This has been used as
counter stain substitute to eosin ( Eman Hashim, 2006, Abdul-Afeez Ibnouf, 2014,
Raheem et al., 2015). It has also been
explored as nuclear stain to substitute for haematoxylin (Benard, 2008, Benard
et al., 2015, Benard et al., 2015, Benard et al., 2016, Muhammed et al., 2016,
Agbede et al., 2017, Benard et al., 2017 and Omorodion and Achukwu, 2017).
These promising efforts should be
followed up for the local laboratories to benefit from the translational
research this findings offer.
References
1. Abd-Alhafeez Ibnouf, Esam AbdulRaheem,
Mohamed Seed Ahmed, and Dalia Dahab (2014). Assessment of staining quality of
Roselle (Hibiscus Sabdariffa) on formalin-fixed paraffin-embedded renal tissue
sections. Int J Cur Res Rev; 6 (21): 26-28.
2. Agbede,
M.B., Benard, S.A., Afolabi, O.O., Okoye, J.O., Bankole, J.K., Fowotade, A.A.,
Olutunde, O.A., Muhammed, O.A (2017): The
Use of Hibiscus Sabdariffa Extract as Nuclear Stain for Skin Morphology and
Connective Tissue with Eosin Counterstain. Sokoto Journal Of Medical Laboratory
Science; 2(4):28-32
3. Al-Amura, M. F.A., Hassen, Z.A. and ALMhanawi,
B.H. (2012). Staining technique
for helminth parasites by use red beet (Beta
Vulgaris L.) extract. Bas. J. Vet.
Res. 11(1):
283-292.
4. Avwioro, O.G., Onwuka, S.K., Moody,
J.O.,Agbedahunsi, J.M., Oduola, T., Ekpo, O.E. and Oladele, A.A. (2007).
Curcuma longa extract as a histological dye for collagen fibres and red blood
cells. J.Anat. 210: 600–603.
5. Bassey
R.B., Osinubi A.A., Oremosu A.A. (2012): Staining effect of Hibiscus sabdariffa
extract on sperm cell morphology of Sprague –Dawley rats. Journal of histotechonology 35(3): 110-113
6. Benard S.A., Muhammed A.O., Fowotade
A.A., Afolabi O.O., and Olutunde O.A.(2017).
Iron Roselle: A Progressive Nuclear Stain For Connective Tissue Of Skin.
International Journal of Health Research and Innovation, vol.5. no.2, 25-31,
ISSN: 2051-5057 (print version). Scienpress Ltd.
7. Benard S.A., Muhammed A.O., Fowotade
A.A., Afolabi O.O., Olutunde O.A. (2015): Sorghum bicolor extract: A Suitable Counterstain In
Hibiscus Extract Nuclear Staining of Liver And Kidney: African Journal Of
Cellular Pathology 4: 13-16
8. Benard S.A., Muhammed A.O., Fowotade
A.A., Afolabi O.O., Olutunde O.A. (2015): Hibiscus Sabdariffa Etract As Haematoxylin Substitute
In The Histological Demonstration Of Brain Tissues: African Journal Of Cellular
Pathology: 5:32-35
9. Benard
Solomon. Iron-Roselle (2008): A Progressive Nuclear Stain Substitute For
Haematoxylin. J. Histotechnologyy. Vol. 31. 2, 31:57-59.
11. Egbujo EC, Adisa OJ, and Yahaya AB. A Study of the Staining
Effect of Roselle (Hibiscus Sabdariffa) on the Histologic Section of the
Testis. Int. J. Morphol. 2008; 26(4):927-930.
12. Eman A Hashim (2006). The use of watery extract of Kujarat
flowers Hibiscus Sabdariffa as a natural histological stain. Iraqi J Med
Sci. 5 (1): 29-33.
13. Gharravi, A.M., Golarlipour, M.J., Ghorbani,
R.and Khazaei, M. (2006). Natural dye for staining astrocytes and neurons. J.Neuro.
Sci. (Turkisk) 23(3): 215-218.
14. Ihuma JO, GH Asenge, JOK Abioye, and SK Dick (2012). Application of Methanolic extracts from
Hibiscus Sabdariffa Linn as a biological staining agent for some fungal
species. International Journal of Plant, Animal and Environmental Sciences.;
2 (2): 254-9
15. Okolie, N.J.C. 2008. Staining of ova of intestinal
parasites with extracts of Hibiscus Sabdariffa and Azadirachta Indica.
Inter. Sci. Res. J. 1(2): 116-119.
16. Muhammed A.O., Olutunde O.A., Benard
S.A., Muhammad Ahmad AT and Omoowo BT (2016):Hibiscus-Shorgum: A New Morphological
Stain in Neuro-Histology: International Journal of Health Research and
Innovation, vol.4. no.1, 31-38 ISSN: 2051-5057
17. Omorodion
N.T. and Achukwu P.U. (2017).
Investigation of Hibiscus Sabdariffa (Roselle) as Histological Stain and
in Assessment of Bar Bodies. American
Journal of Biomedical Sciences; 9(1), 15-19.
18. Raheem M.A., Abd-Alhafeez O.I., Osman H.S., Hamza J.M
(2015). Using of Hibiscus Sabdariffa extract
as a natural histological stain of the Skin. American Journal of Research
Communication; 3(5), 211-216.
19. Samanta, A. K., Agarwal, P. and Datta, S.(2007).
Dyeing of jute and cotton fabrics
using jackfruit
wood extract: Part-I :Effects of mordanting and dyeing process variables on
colour yield and colour fastness properties. Indian J Fibre &
Text Res. 32(12): 466-476.
20. Suabjakyong, P., Romratanapun, S. and Thitipramote,
N. (2011). Extraction of natural histological dye from black plum fruit (Syzygium
cumini). J.Microsc. Soc. Thailand. 4(1): 13-15.
21. Suebkhampet, A. and Sotthibandhu, P. (2012).
Effect of using aqueous crude extract from butterfly pea flowers (Clitoria
ternatea L.) as a dye on animal blood smear staining.
22. Tousson, E. and Al-Behbehani, B. (2011). Black Mulberries (Morus nigra) as a
natural dye for animal tissue staining. Animal
Biol. 62(11): 49-56.
23. Umar, A.A., Hena, S.A. and Wiam, I.M. (2012).
Kola nut (Cola acuminata) extract as a substitute to histological tissue
stain eosin. Sci. J. Vet. Adv. 1(2):33-37.